정유석·양원용·홍성표·박
준*·이해열**·유원영**·강수형**
경희대학교
의과대학 성형외과학교실, 포천중문의과대학 분당차병원
성형외과학교실*,
동아제약연구소**
In
Vivo Study of the Chitosan-Cross-Linked Collagen-Glycosaminoglycan
Dermal Substrate.
Department of Plastic and
Reconstructive Surgery, College of Medicine, KyungHee
University, Department of Plastic and Reconstructive
Surgery, College of Medicine, Pochon CHA University,
Bundang CHA General Hospital*, Research Lab, Dong-A
Pharmaceutical Co. Ltd**.
The
treatment for full thickness skin defect with the
full or split-thickness autograft was often associated
with aesthetically poor result, hypertrohic scar
or extensive injury on donor site. Because of this
donor morbidity, the use of artificial dermis was
considered, which was the substrate of collagen
or collagen cross-linked with polyglactin or polylactin.
Unfortunately this substrate was void of advantages
for the artificial dermis but was of easy biodegradability,
antigenicity, or cytotoxic property.
Currently
chitosan is given attention by many investigators
for fts biochemical properties in wound healing
process. Chitosan is the deacetylated derivative
of chitin, which is a polymer of 2-amino-2-deoxy
glucose with chemically active free amino group.
Chitosan facilitates wound healing process, and
then stimulates migration of polymorphonuclear cell(PMN)
and macrophage, release of IL-8 and accelerates
collagen synthesis surely with vascularization.
Indeed chitosan supplies the resistance against
bacterial infection of the wound.
In
this study, we have investigated the clinical applicability
of the artificial dermal substrate which is cross-linked
of collagen and GAG with chitosan, which we have
applied on the full thickness skin defect in Fisher
rat.
The
conclusions are as the follows:
1.
There was sufficient vascularization in the grafted
dermal substrate for STSG after 2 weeks of artificial
demlis grafting.
2.
Four 4 weeks after artificial dermis grafting, the
architecture of the dermal substrate was maintained
in about half amounts and the half of dermal skeleton
was replaced with the newly formed dermis(neodermis).
In
conclusion, the dermal substrate used in this study
is available enough for wound of full thickness
skin defect.
Key
Words: Chitosan, Artificial dermal substrate |
