양원용
. 서상우 . 홍성표 . 김홍석 . 이두성 . 최귀원 . 배태수
겅희대학교
의과대학성형외과학교실, 성균관대학교 고분자공학과,
한국과학기술연구원
A
Study on the Optimal Culture Condition and Biomechanical
Properties of
In Vitro Tissue Engineered Cartilage
Using a Fibrin Glue Scaffold
Won Yong Yang M.D.
Sang Woo Suh, M.D., Sung Pyo Hong, M.D., Hong
Suk Kim M.D.
Doo Sung Lee M.D. Kui Won Chol,
M.D. Tae Soo Bae, M.D.
The
purpose of this study was to examine the feasibility
of using fibrin glue polymer designed to produce
tissue-engineered cartilage by in vitro culture
and to determine the optimal fibrinogen and chondrocyte
concentrations required to generate cartilage and
to evaluate the biomechanicaI properties of the
generated cartilages. The most favorable fibrinogen
and chondrocyte concentrations were determined by
measuring the volume and weight gains, evaluating
the histologic changes and measuring
the biomechanical properties after construction
of l2 different chondrocyte-fibrin glue constructs.
The
results are as follows.
1.
The fibrin glues without chondrocytes degraded during
8 weeks of culture period.
2.
The construct gains more weight in those with higher
chondrocyte and fibrinogen concentration.
3.
Histologic analysis of the generated cartilage showed
nearly homogeneous cartilage when
using 80mg/cc fibrinogen and 4 × chondrocyte/cc
at 8 weeks in vitro culture.
4.
The compressive modulus is higher in constructs
with higher fibrinogen concentration and is 35.4
- 36.8% of the normal joint cartilage.
5.
The yield stress is higher in constructs with higher
fibrinogen concentration and is 11% of the normal
joint cartilage
This
study demonstrates that fibrin glue is a suitable
polymer for generation of cartilage by in vitro
culture method. The construct with 80mg/cc fibrinogen
and 4× chondrocyte/cc yields the best quality
of cartilage at 8 weeks when analyzed by weight
and volume changes, histology and biomechanical
properties. The compressive modulus and yield stress
of the generated cartilage are lower than those
of normal joint cartilage, and may still be adequate
for the purpose of craniofacial reconstruction which
seeks more pliant cartilage not requiring weight
bearing strength.
Key
Words: Fibrin glue polymer, Tissue-engineered cartilage,
Biomechanical properties |
