이무형,
김휘준, 이진우, 김영일 Korean J Physiol Pharmacol
2002년 6권 5호 275 ~ 279
Nerve
growth factor (NGF) is an important autocrine growth
factor and also a survival factor for keratinocytes.
NGF may act in the hyperproliferative condition,
psoriasis. Clinically, the combination of psoralen
and UVA ( PUVA) has been used in the treatment of
a wide variety of cutaneous disorders, such as psoriasis
and vitiligo. However, the precise therapeutic mechanism
of PUVA on the dermatologic diseases remains unclear.
The purpose of this study was to examine whether
the expression of NGF in cultured keratinocytes
is influenced by PUVA. Thus, normal human keratinocytes
were isolated from neonatal foreskin, and the third
to fifth-passaged cells were used in this study.
The cells were exposed to various doses of UVA (30,60,120
mJ/㎠) after adding 8-methoxypsoralen (8-MOP) to
examine the expression of NGF mRNA. The RNA and
protein of the cells were extracted at various time
points (1, 8, 24 hours) after UVA irradiation to
examine the expression of NGF mRNA and production
of NGF protein. In keratinocytes, there were no
differences in the expression of NGF mRNA between
the different doses of UVA irradiation, however,
the expression of NGF mRNA in UVA and PUVA groups
tended to increase as the time increased. The expression
of NGF mRNA was the highest in PUVA group, followed
by UVA group and the lowest in 8-MOP group. 1 and
8 hours after UVA irradiation were lower in the
PUVA group than in the other groups. This study
showed that the expression level of NGF protein
in keratinocytes was relatively lower in the PUVA
groups than in the other groups, suggesting that
the therapeutic mechanism of PUVA in psoriasis is
related to the decrease of NGF protein. |
