경희대학교
외과대학 응급의학교실 및 약리학교실*
강원대학교
의과대학 약리학교실**, 병리학교실***, 및 해부학교실****
정성구·장현용**·김명천·고영관·정주호*·배영미***,
박원서***,김대중****·유영민**·김성수**·임성빈**
Effects
of Exogenou Nitric Oxide on the Ischemic Damage of H9c2 Cardiac Myoblast Cells
Sung
Koo Jung, M.D., Hyun Yong Jang*, Myung Chun Kim, M.D., Young Gwan Ko, M.D., Joo
Ho Chung, M.D.*, Young Mee Bae, Ph.D.***, Weon Seo Park, M.D.***, Dae Joong Kim
M.D.****, Young Min Yoo**, Sung Soo Kim, M.D.**, Sung Vin Yim M.D,**
Department
of Emergency Medicine and Pharmacology*, College of Medicine, KyungHee University
Department
of Pharmacology**, Pathology*** and Anatomy****,
College of Medicine, Kangwon National
University**
Background:
Nitric Oxide(NO) is known to have protective effects on an ischemic heart and to
exert triggering effects on ischemic preconditioning. However, the effects of NO
during the ischemic period have not been investigated. To investigate the role of
exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects
of ischemic preconditioning and ischemia in a normal and an ischemic buffer.
Methods:
Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered
medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator
with GasPak for 5 hrs. In ischemic preconditioning. the cells were pretreated with
ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the
measurement of the cytotoxicity, a MTT(3-4-5dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium
bromide) assay was performed. A DAPI(4', 6-diamidino-2-phenylindole dihydrochloride)
staining procedure and a flow cytometry analysis were performed to confirm apoptotic
cell death by ischemia
Results:
Cel1 viability. its determined by using a MTT assay. showed that the preconditioned
group treated with NO showed more cell death than with the not-preconditioned groups
in both normal and ischemic buffers. But. In normal medium and not-preconditioned
groups, NO showed protective effect according to the concentrations(100, 1000 uM).
No treatment with NO produced the different results. In normal medium, the protective
effect of ischemic preconditioning was demonstrated, but no protective |
