대피지
2002년 40권 11호 1353 ~ 1358
Background:
Nerve growth factor(NGF) is a neurotrophic polypeptide necessary for the survival
and growth of some central neurons, as well as sensory afferent and sympathetic
neurons. In addition to its actions on t he nervous system, it also has significant
biologic effects on cells of the immune-inflammatory compartment. NGF can variably
induce melanocyte mitosis, increase melanogenesis, enhance dendricity and prevent
apoptotic cell death following the UV injury.
Objective:
The purpose of this study is to evaluate the effects of NGF on proliferation of
cultured human melanocytes.
Methods:
The effects of NGF on proliferation of cultured human melanocytes were evaluated.
To specify the NGF effect on proliferation of human melanocytes, excess of anti-NGF
neutralizing polyclonal antibody was added.
Results:
1)
In 1% of melanocyte growth supplement(MGS) medium, all experimental groups showed
no significant increase of cell number. The addition of anti-NGF polyclonal antibody
did not influence the proliferation of melanocytes.
2)
In 0.2% of MGS medium, all experimental groups showed no significant increase of
cell number. The addition of anti-NGF polyclonal antibody did not influence the
proliferation of melanocytes.
Conclusion:
These results suggest that, even though NGF has many biological influencse on melanocytes,
it does not play a significant role in the proliferation of melanocyts. |
