대피지
2002년 40권 4호 375 ~ 385
It
is known that skin diseases are related to proliferation of cellular components.
Green
tea has many favorable biologic effects: anti-inflammatory, antibacterial, antiviral
effects, lowering of plasma cholesterol and triglyceride levels, reduction of blood
pressure and platelet aggregation, antimutagenic, and anticarcinogenic activities.
The effects of green tea on various cells in skin has been reported in previous
studies; For keratinocytes and human epidermoid carcinoma cells, green tea induces
apoptosis only in cancer cells, but no keratinocytes. For endothelial cells, green
tea inhibits endothelial cell proliferation. For melanocytes and fibroblast, it
is little known.
We
investigated the effects of green tea polyphenol(GTP) and the major constituents,
(-)-epigallocatechin-3-gallate(EGCG) on the proliferation in the cultured human
keratinocytes, fibroblasts, melanocytes, endothelial cells and human epidermoid
carcinoma cells(A431 cells). The proliferative response was studied by the cell
count and the uptake of tritiated thymidine after 48 hours of treatment. And we
also observed apoptosis using TUNEL staining.
The
results were as follows; Number of living cells were significantly decreased(p<0.05)
in cultured human epidermal cells, melanocytes, fibroblasts, endothelial cells and
A431 cells treated with different concentration of GTP and EGCG for 48 hours. The
cells were decreased dose dependently as increase of concentration of the extracts.
The [3H]thymidine incorporation were significantly decreased(p<0.05) in cultured
human epidermal cells, melanocytes, fibroblasts, endothelial cells and A431 cells
treated with different concentration of GTP and EGCG for 48 hours. The dose dependent
inhibition was also seen in [3H]thymidine incorporation by green tea extracts. Cell
proliferation was significantly more inhibited(p<0.05) by EGCG compared to GTP
in the same concentration. We failed to observe apoptosis of cultured human epidermal
cells, melanocytes, fibroblasts, endothelial cells and A431 cells by GTP and EGCG.
In
summary, green tea extracts(GTP, EGCG) showed statistically significant inhibitory
effects on cultured human epidermal cells, melanocytes, fibroblasts, endothelial
cells and A431 cells. These results suggest that green tea could play a possible
role in the treatment of hyperproliferative skin disease. |
