대피지
2001년 39권 4호 435 ~ 445
Background：Psoriatic
keratinocytes express CXC chemokines like IL-8 and GRO-α, and CC chemokines like
MCP-1 and RANTES, which have a significant role in the accumulation of inflammatory
cells in psoriatic skin and both CXCR1 and CXCR2 receptors are also expressed in
psoriatic keratinocytes, which suggests that IL-8 and GRO-α could have a role in
the characteristic epidermal changes through binding to their receptors in psoriatic
keratinocytes. Objective：The purpose is to understand the pathogenetic mechanisms
of psoriasis by comparing immunoreactivity of various chemokines and chemokine receptors
between lesional and non-lesional skin of psoriasis. Methods：We have performed
immunohistochemical studies with mouse anti-human IL-8, mouse anti-human GRO, anti-huamn
MCP-1, mouse anti-human RANTES, anti-human CDw 128 IL-8RA/ CXCR1, and anti-human
IL-8RB/CXCR2 for lesional and non-lesional skin of ten psoriatic patients. Results：
1. Immunohistochemical reactivity for IL-8 is stronger in lesional epidermis than
non-lesional epidermis(p<0.05) and immunohistochemical reactivity for GRO-α
is stronger in lesional epidermis than non-lesional epidermis(p<0.05). 2. Immunohistochemical
reactivity for MCP-1 is stronger in lesional epidermis than non-lesional epidermis(p<0.05),
and immunohistochemical reactivity for RANTES is stronger in lesional epidermis
than non-lesional epidermis(p<0.05). 3. Immunohistochemical reactivity for CXCR1
is stronger in lesional epidermis than non-lesional epidermis(p<0.05) and immunohistochemical
reactivity for CXCR2 is stronger in lesional epidermis than non-lesional epidermis(p<0.05).
4. Immunofluorescent staining reveals positive finding in epidermis of lesional
psoriasis, but negative finding in CXCR2 Conclusion：These results suggest that
psoriatic keratinocytes express CXC chemokines like IL-8 and GRO-α, and CC chemokines
like MCP-1 and RANTES, which have a significant role in the accumulation of inflammatory
cells in psoriatic skin and that both CXCR1 and CXCR2 receptors are also expressed
in psoriatic keratinocytes, which suggests that IL-8 and GRO-α could have a role
in the characteristic epidermal changes through binding to their receptors in psoriatic
keratinocytes. |
