박지용
이무형 허충림 경희의학 2000:16:1 23-29
Background: Human growth hormone (hGH) plays a central
role in linear bone growth and body metabolism.
According to "somatomedin hypothesis",
many GH-mediated effects are exerted systemically
via stimulation of hepatic synthesis of insulin
like growth factor 1 (IGF-1). However, due to discovery
of growth hormone receptor in peripheral tissue,
GH can be thought to act not tony via systemic IGF-1
but also directly via growth hormone receptor expressed
on many target tissue. Objective: We attempted to
evaluate the effect of hGH on proliferation of different
passaged cultured human fibroblast. Methods: We
investigated hGH effect on fibro-blastl proliferation
by [3H] thymidine incorporation. Results : The results
were as follows; 1. There was a significant increase
(p<0.05) of [3H] thymidine incorporation in 5,
10, 15 passaged cells by adding hGH (10ng/mL) 2.
There was a significant inhibition (p<0.05) of
decrease of [3H] thymidine incorporation in 15 passaged
cells by adding hGH (10ng/mL) Conclusion: Our results
showed the possibility of direct proliferation and
anti-aging effects of the hGH on cultured human
fibroblast. |
